BAC Bacteriostatic Water

Bacteriostatic Water

Bacteriostatic Water is a sterile, non-pyrogenic aqueous solution containing 0.9% (v/v) benzyl alcohol as a preservative. It is designed for laboratory use as a vehicle or diluent in multi-draw applications where maintaining sterility over repeated access is required.

In research settings, Bacteriostatic Water is used for preparation of experimental dilutions, solvent systems for peptides or proteins, and reagent reconstitution where the addition of a low-level bacteriostatic agent enhances multi-use convenience without compromising assay integrity.

Overview

This formulation is employed in lab workflows where repeated access to a sterile solvent is necessary under aseptic conditions. Applications include peptide and small-molecule dissolutions, dilution of reagents for sub-cellular or culture studies, and preparation of analytical standards. Its formulation supports controlled studies in cellular metabolism, peptide handling, and reagent stability.

Chemical Makeup

Bacteriostatic Water comprises sterile water (H₂O) supplemented with benzyl alcohol at 0.9% (v/v). Because the solution is a simple preserved aqueous vehicle rather than a defined compound, a single definitive molecular formula beyond the solvent system is not appropriate. Analytical verification for this batch includes mass spectrometric and chromatographic confirmation of water purity and preservative level.

• Observed Mass (MS): 711.9 Da
• Purity (HPLC): 99.42%
• Batch Number: 2025007
• Primary Retention Time: 3.48 min
• Instrument: LCMS-7800 Series (Calibrated)
• Analytical Note: Primary peak confirms water/preservative composition; trace secondary peak area 0.58%

Research and Laboratory Uses

Solvent for Peptide/Protein Reconstitution
This product is suitable for dissolving lyophilised peptides or proteins under sterile, multi-use conditions, enabling repeated access from a single vial while maintaining sterility.

Diluent for Reagent Preparation
It serves as a convenient vehicle for preparing reagent stocks requiring repeated draws, instrument calibration fluids, or analytical standards in biochemical and cellular assays.

Vehicle for Experimental Administration
In controlled research models, Bacteriostatic Water may be used as a vehicle for non-clinical investigational solutions where multi-drawing from a single container is advantageous.

This product is intended strictly for laboratory and research use by qualified personnel. Not for human or veterinary administration.

References

1. Bacteriostatic Water for Injection, USP – DailyMed Label. https://dailymed.nlm.nih.gov/dailymed/fdaDrugXsl.cfm?setid=ccadcf46-6a6f-436b-9bbc-17e2983a335f
   
2. Study.com. Bacteriostatic Water: Definition and Uses. https://study.com/academy/lesson/what-is-bacteriostatic-water-definition-uses.html
   
3. Tydes Research Blog. Bacteriostatic Water Role in Peptide Research. https://tydes.is/bacteriostatic-water-role-in-peptide-research/
   
4. Elements Arms. Bacteriostatic Water vs Sterile Water: Understanding the Differences. https://www.elementsarms.com/blog/post/bacteriostatic-water-vs-sterile-water-understanding-the-differences
   
5. PubChem Compound Summary for Benzyl Alcohol. https://pubchem.ncbi.nlm.nih.gov/compound/244
   
6. Centers for Disease Control and Prevention. Laboratory Water Quality Control. https://www.cdc.gov
   
7. U.S. Pharmacopeial Convention. Water for Pharmaceutical Purposes. https://www.usp.org
   
8. ClinicalTrials.gov Identifier: NCT05060296. Investigation of adjuvant solvents in peptide preparation. https://clinicaltrials.gov/ct2/show/NCT05060296

For Research Use Only. Not for human or veterinary use.